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Reactivity | Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
Calculated MW | 112kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human NEFH |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | NFH; |
Western blot analysis of extracts of mouse brain cells, using NEFH antibody.
Immunohistochemistry of paraffin-embedded mouse kidney using NEFH antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat kidney using NEFH antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using NEFH antibody at dilution of 1:100 (x40 lens).
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
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